CCL:G: Energy barriers-BLYP method

 Sent to CCL by: Nagesh Babu Idupulapati [nbi002*_*]
 Dear CCL members
 I am performing Transition state calculations on a particular enzyme oxidation
mechanism by obtaining reactant, intermediates and product using gaussian calculations using BLYP/6-31G** method and basis set.
Iam getting energy barriers between 20-30 kcal/mol between different intermediates and transition states involved in the mechanism. Actually, there is no experimental evidence for me to compare these barriers for that particular enzyme.
can anybody please let me know, whether the energy barriers obtained by this BLYP are underestimated or overestimated?????
 I really need to justify my results, if they are ok
 I would really appreciate anybody's help.
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