Summary: Phospholipid 3d structure and forces



 Hi!  Here is what I have heard/found about Phospholipids 3d structures
 and forces/characteristics (which I need find values for each characteristic
 of membrance components).  The idea is to model a Cell (to be freely
 available for non-profit research).
 (Also I am working on writing a Virtual Cell Bio. Course that will be
  available via WWW/Mosaic - I need images still, clickable biology).
 I hope this can be of help to others also.
 Thanks!
 Mark
 Original Post:
  Subject: CCL:Phospholipid 3d structure and forces
  Date: Tue, 29 Nov 1994 17:19:16 -0600 (CST)
 Hi!  I was wondering if anyone has suggestions or know of places where I
 could get 3D structures (PDB format) of (cell membrane) phospholipids,
 also if there are any formulas/information on strengths and rate of
 decrease of the hydrophilic/phobic interactions, pH and ionic strength
 of various phospholipids, proteins.
 	Basically, I am looking for good, accurate and complete information
 of characteristics/properties of the various parts of cell membranes.
 Does anyone have good references?
 ---------------------< End of message >------------------------------
 Just so it is clear, I am doing this on my own time, i.e. it is not
 part of my job.   So Cray is not responsible for my actions,comments,
 or ideas.
 	Of course the Biochem. and Cell Bio. textbooks.
 Here is what I have found (in the Cray Channels Volume 16, Number 1):
 Terry Stouch's work:
      1. Stouch, Terry R., "Lipid Membrane Structure and Dynamics Studied by
 	All-Atom Molecular Dynamics Simulations of Hydrated Phospholipid
 	Bilayers"  Molecular Simulations, Vol. 10, No 2-3. pp. 317-345.
      2. Stouch, Terry R., Howard E. Alper and Donna Bassolino,
 "Supercomputing
 	Studies of Biomembranes" International Journal of Supercomputer
 	Appllications, 1993.
      3. Alper, Howard E., Donna Bassolino, and Terry R. Stouch, "Computer
 	Simulations of a Phospholipid Monolayer-Water System: The influence
 	of Long Range Forces on Water Structure and Dynamics"  Journal of
 	Chemical Physics, Vol 98, No. 12, pp. 9798-98-7, 1993.
      4. Alper, Howard E., Donna Bassolino, and Terry R. Stouch, "The
 Limiting
 	Behavior of Water Hydrating a Phospholipid Monolayer: A Computer
 	Simulation Study," Journal of Chemical Physics, Vol. 99, No. 7,
 	pp. 5547-5559, 1993.
      5. Stouch, Terry R., Keith B. Ward, Amanda Altieri, Arnold T. Hagler,
         "Simulation of Lipid Crystals: Characterization of Potential Energy
 	Functions and Parameters for Lecithin Molecules," Journal of
 	Computational Chemistry, Vol. 12, No. 8, pp. 1033-1046, 1991.
      6. Stouch, Terry R. and Donald E. Williams, "On the Conformational
 	Derived Charges: Studies of the Fitting Procedure," Journal of
 	Computational Chemistry, Vol. 14, No. 7, pp. 858-866, 1993.
      7. Liang, Congxin, Carl S. Ewig, Terry R. Stouch, and Arnold T. Hagler,
 	"Ab Initio Studies of Lipid Model Species. 1. Dimethylphosphate and
 	Methylpropylphosphate Anions," Journal of American Chemical Society,
 	Vol. 115, No. 4, pp. 1537-1545, 1993.
      8. Stouch, Terry R. and Donald E. Williams, "Conformational Dependence
 	of Electrostatic Potential Derived Charges of a Lipid Headgroup:
 	Glycerylphosporylcholine," Journal of Computational Chemistry, Vol. 13,
 	No. 5, pp. 622-632, 1992.
      9. Williams, Donald E. and Terry R. Stouch, "Characterization of Force
 	Fields for Lipid Molecules: Applications to Crystal Structures,"
 	Journal of Computational Chemistry, Vol. 14, No. 8, 1993.
     10. Bassolino, Donna, Howard E. Alper, and Terry R. Stouch, "Solute
 	Diffusion in Lipid Bilayer Membranes: An Atomic Level Study by
 	Molecular Dynamics Simulation," Biochemistry, Nov. 30, 1993.
 Responses:
 1. From mchee : at : oxmol.com Wed Nov 30 08:53:54 1994:
    Oxford Molecular has a package called Iditis which validates the PDB files
    from BrookHaven and derives additional properties for each protein (from
    macromolecular to atom level information).  Thus, users will be able to
    obtain all sequence and structurel related  information about any protein
    in the database.
    Anyway, in the latest release of the data, which corresponds to the April
    1994 release of Brookhaven, there are 4 entries of the same phospholipid
    protein - 1AIN (Annexin I from Human), 1ALA (Annexin V from chicken), 1AVH
    (Annexin V Hexagonal crystal), 1AVR(Annexin V Rhombohedral crystal). The
    last two are from human placenta.  All are Ca-binding.
    If you are interested in getting more information about Iditis, please
    contact the number below.
    Regards,
    Merk-Na
    -------------------------------------
    Merk Na Chee, Ph.D.
    Applications and Support
    Oxford Molecular Inc.
    Springfield, VA 22151
    E-mail: mchee : at : oxmol.com (Merk Na Chee)
    -------------------------------------
 2. From DCAMPER : at : elinet1.dowelanco.com Wed Nov 30 06:31:29 1994
    Subject: lipids
    I don't have any specific reference at hand but I know
    Terry Stouch at Bristol Meyers Squib was involved in
    extensive modeling of lipid bylayers and I believe molecules
    within these lipids.  This may be a place to start.
    Debby Camper
    dcamper : at : dowelanco.com
 3. From ccevc16 : at : pinar1.csic.es Wed Nov 30 02:38:08 1994
    From: Victor Cruz <ccevc16 : at : pinar1.csic.es>
    Subject: Phospholipids
    You can find geometrical information at the Cambridge Crystallographic
    Database. In particular I am working with DLPE
    (dilauroil-phosphatidyl-choline) crystal structure taken from:
    Hitchcock,P.B.,et al.,Proc. Nat. Acad Sci. 71:3036(1974).
    Cambridge Database can be get from: Dr. Olga Kennard
    CCDC,University Chemical Laboratory,Lensfield Road,Cambridge CB2 1EW,U.K.
 4. From newhoir : at : duc.auburn.edu Tue Nov 29 18:03:54 1994
    From: newhoir : at : duc.auburn.edu (Irene Newhouse)
    Subject: Re: CCL:Phospholipid 3d structure and forces
    There's a big review on phospholipid structures by Irmin Pascher in
    Biochimica et Biophysica Acta that came out at the end of 1992; vol 1113,
    pp 339-73.
    Irene Newhouse
 5. From wchutt : at : alex.monsanto.com Thu Dec  1 15:02:15 1994
    From: wchutt : at : alex.monsanto.com (Bill C Hutton)
    Subject: phospholipds
    The only 3D data I know of is an old X-ray structure. I do not have the
    referene here, but I could bring it in. This is a very well known
    paper and is used in all of the monographs and reviews of phospholipid
    membranes. Some simulation work (computational chemistry) has been done
    as well.
    Also, I have dozens of non-propriatary NMR NOESY spectra of
    phospholpipd vesicles. No one really understands some of the featurews
    in this data. Modeling could really help out. This data could go a long
    way towards understanding curved bilayer structure and dynamics. I have
    not had the time or incentive to carefully evaluate all of the data.
    But is is quite interesting. Some early work can be found in J. Amer.
    Chem. Soc. 1985, 107 pp 1530-37. There are later papers as well but
    again I do not have the references here.
    **********************************************************************
    W.C. Hutton, Monsanto Company, Corporate Research
    wchutt : at : monsanto.com
    This message does not necessarily reflect the views of Monsanto Company
    or, now that I think of it, anyone else.
    **********************************************************************
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