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QCMP127. SILMUT: A Program for the Identification of
Regions Suitable for Silent Mutagenesis to Introduce
Restriction Enzyme Recognition Sequences
by Basavaraju Shankarappa, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15281 This package, consisting of the two programs TABLE and SILMUT, is capable of selectively identifying potential sites for silent mutagenesis within a target DNA sequence. It is based on a strategy of identifying amino acid motifs compatible with each restriction site. The programs can be used to identify the suitability for the introduction of any 6-base nucleic acid sequence such as restriction enzyme sites in casette mutagenesis strategies. The TABLE program generates a table of multiple amino acid motifs for each restriction enzyme obtained by translating each unique recognition sequence in all three reading frames. The SILMUT program utilizes features of TABLE and can further identify the presence of a match between any amino acid motif of each restriction enzyme and the input target sequence. Lines of Code: 1523 C |